This animal study was conducted by researchers at Ohio State University and presented at Neuroscience 2023, the annual meeting of the Society for Neuroscience. (
A surprising thing happened when researchers began exploring whether early-life stress compounds the effects of a childhood head injury on health and behavior later in life. It’s already known that head injuries are common in young kids, especially from falling.
“We wanted to understand whether experiencing a traumatic brain injury in the context of early life stress circumstances could modulate the response to the brain injury. And using an animal model allows us to really get into the mechanisms through which these two things might be impacting brain development as it’s occurring,” said senior study author Kathryn Lenz, associate professor of psychology at The Ohio State University.
This first set of experiments in rats suggests early life stress’s potential to lead to a lifetime of health consequences may not be fully appreciated, Lenz said.
“We found many, many, many more genes were differentially expressed as a result of our early life stress manipulation than our traumatic brain injury manipulation,” Lenz said. “Stress is really powerful, and we shouldn’t understate the impact of early life stress on the developing brain. I think it tends to get dismissed – but it’s an incredibly important public health topic.”
Researchers temporarily separated newborn rats from their mothers daily for 14 days to induce stress mimicking the effects of adverse childhood experiences, which include a variety of potentially traumatic events.
On day 15, a time when rats are developmentally equivalent to a toddler, stressed and non-stressed rats were given either a concussion-like head injury under anesthesia or no head injury. Three conditions – stress alone, head injury alone, and stress combined with head injury – were compared to uninjured, non-stressed rats.
First author Michaela Breach, a graduate student in Lenz’s lab, examined the gene expression changes in the hippocampal region of the animals’ brains later in the juvenile period using single-nuclei RNA sequencing.
Stress alone and stress combined with traumatic brain injury (TBI) produced a few noteworthy results. Both conditions activated pathways in excitatory and inhibitory neurons associated with plasticity, which is the brain’s ability to adapt to all kinds of changes – mostly to promote flexibility, but sometimes, when the changes are maladaptive, resulting in negative outcomes.
“This may suggest that the brain is being opened up to a new period of vulnerability or is actively changing during this period of time when it could program later life deficits,” Breach said.
Both conditions also had an effect on signaling related to oxytocin, a hormone linked to maternal behavior and social bonding. Stress alone and combined with TBI activated this oxytocin pathway, but brain injury alone inhibited it.
“Both stress and TBI are linked to abnormal social behavior, but we’re finding these differing effects with the oxytocin signaling,” Breach said. “That demonstrates that the effect of stress might modulate how TBI changes the brain since the combination treatment was different from TBI on its own. Oxytocin is involved in the response to stress and repair, so that may mean it could be an interesting modulator for us to pursue in the future.”
In behavior tests in rats that had aged into adulthood, only animals that experienced early-life stress were prone to more frequently entering a wide-open space – a location that typically makes rodents feel vulnerable to predators.
“Overall, that suggests they might be taking more risks later in life, which is consistent with human data showing that early life stress can increase the risk for certain conditions like ADHD, which can be characterized by risk-taking behavior or substance use disorders,” Breach said.
The behavior data pointing to detrimental effects of early-life stress provides further evidence of the need to address adverse childhood experiences, Lenz said.
- Examining the impact of early life stress and pediatric TBI on the developing hippocampal transcriptome and behavioral development in rats. – (https://www.abstractsonline.com/pp8/#!/10892/presentation/29611)